Comparison of panbio dengue duo igm and igg capture elisa and venture technologies dengue igm and igg dot blot. Antibodies to dengue if present in the specimen, will bind to the antihuman igg antibodies adsorbed onto the surface of the wells. The panbio dengue igm capture elisa is for the qualitative detection of igm antibodies to dengue antigen in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms consistent with dengue fever. Request pdf comparison of panbio dengue duo igm and igg capture elisa and venture technologies dengue igm and igg dot blot a number of commercial. A commercially available capture enzymelinked immunosorbent assay elisa for the detection of specific immunoglobulin m igm and igg antibodies produced during dengue infection panbio dengue. The panbio dengue igg capture elisa is for the qualitative presumptive detection of elevated igg antibodies to dengue virus serotypes 14 in patients with. Pdf the objectives of this study were to conduct a further evaluation of.
Comparison of panbio dengue duo igm and igg capture elisa. Evaluation of capture elisa and rapid immunochromatographic. I use a capture elisa kit from panbio that works great in detecting human anti dengue igg. Comparison of performance of serum and plasma in panbio. Test procedures and interpretation of results were carried out in accordance with the manufacturers instructions. Introduction dengue, a flavivirus, is found in large areas of the tropics and subtropics. Seven commercial assays were evaluated to determine their suitability for the diagnosis of acute dengue infection. Commercial tests, namely, the panbio dengue igm capture elisa system panbio, sinnamon park, australia and pathozyme dengue m omega diagnostics, alva, uk, were performed according to the manufacturers protocols.
Not for sale or distribution in the united states of. An antigen capture enzymelinked immunosorbent assay reveals high levels of the dengue virus protein ns1 in the sera of infected patients. We base our values on how high over background values that the antibodies detect at. The focus diagnostics dengue virus igm capture dxselecttm is intended for the detection of human serum igm antibodies to dv types 14. Detection of specific antibodies in saliva during dengue. A previous evaluation of available tests for igm antidenv. Evaluation of commercially available diagnostic tests for the. Mean differences for serum and plasma panbio units was generally small ranging from 0.
Cross reactivity is seen in the flavivirus group between dengue virus, murray valley encephalitis. Evaluation of commercially available antidengue virus. Comparison of panbio dengue duo enzymelinked immunosorbent. Panbio dengue igm capture elisa rapid point of care diagnostics. Comparison of two generations of the panbio dengue.
Dengue virus elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Anti dengue specific igg present in the serum sample was assayed according to the. It may also be used to distinguish between primary and secondary. Apr 02, 2010 video describing the steps undertaken in performing elisa to detect igm antibodies against dengue in patient serum. Saliva was collected prospectively from patients presenting with suspected dengue infection 4 to 8 days after the onset of symptoms and assayed by a commercial dengue immunoglobulin m igm and igg capture enzymelinked immunosorbent assay elisa panbio dengue duo elisa. Details of the supplier of the safety data sheet manufacturersupplier. Platelia dengue igg capture is designed to detect ns1 specific igg useful to differentiate primary and secondary dengue virus infections 6, 7. Transmission is by mosquito, principally aedes aegypti and aedes albopictus. Specimens and controls are added to the microtiter wells and incubated. The panbio igg capture elisa correctly classified 67% of secondary dengue infection cases. Comparison of panbio dengue igm elisa assay with pentax dengue igm. Dengue igg microlisa test is an enzyme immunoassay based on gaccapture elisa.
Comparison of two generations of the panbio dengue ns1. Dec 29, 20 none of the 43 confirmed non dengue cases and 20 healthy individual samples were positive by ns1 elisa giving specificity of 100%. This assay doesnt detect low level of dengue igg ab present from past infection. All the samples were then subjected to dengue igmcapture elisa by inbios igm antibody capture macelisa, seattle, usa. Evaluating the sensitivity of a commercial antigen capture. Use of a rapid test kit and elisa for detection of ns1. Comparison of a dipstick dotelisa with commercial assays for. Panbio dengue igg indirect elisa wash buffer, sample diluent, hrp conjugate 1. Paired sera from patients with dengue n 20 and japanese encephalitis je, n 10, and single sera from patients with typhoid n 10, leptospirosis n 10 and scrub typhus n 10 were. The venture technologies dengue igm and igg dot blot assays and the panbio dengue duo igm and igg capture elisa were compared.
Pdf evaluation of the mrl diagnostics dengue fever virus. Enhanced performance of an innovative dengue iggigm rapid. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. International external quality control assessment for the serological. Dengue igmcapture elisa panbio dengue iggindirect elisa panbio dengue ns1 antigen detection elisa panbio trainer. If both ns1 and igm positive results were combined, the detection rate increased to 89. Laboratory diagnosis was based on virus isolation and on hemagglutination inhibition hai assay and an inhouse igm and.
Relevant identified uses of the substance or mixture and uses advised against not available not available address. The drde dengue dipstick dot elisa was performed on the basis of the dot elisa principle. Evaluation of capture elisa and rapid immunochromatographic test for determination of igm and igg antibodies produced during dengue infection. In the panbio dengue duo igm and igg capture elisa, two microtitre plates are supplied, one containing stabilised dengue 14 antigens and the other containing either antihuman igm or antihuman igg bound to separate microwells. The mrl elisa detects only igm for the diagnosis of active. Paired sera from patients with dengue n20 and japanese encephalitis je, n10. Panbio dengue igm capture elisa wash buffer, sample diluent, antigen diluent buffer, dengue 14 antigen, hrp conjugate 1. Sera from hospital attendees, with requests for denv antibody testing, were tested with the panbio dengue duo cassette and a reference antibody capture assay for the detection of igm dengue igm capture elisa national institute of virology, india and igg dengue igg capture. The panbio dengue igm capture elisa should be used in conjunction with other dengue serology. Diagnosis of dengue by igm capture elisa with principle, procedure and result interpretation description of the test. Dengue virus infection causes a spectrum of clinical manifestations ranging from unapparent to fatal haemorrhagic disease. Platelia dengue igg capture is an immunocapture assay designed to detect the specific high level of dengue igg. We evaluated two new commercial dengue diagnostic tests, the mrl diagnostics dengue fever virus igm capture elisa and the panbio rapid immunochromatographic test, on serum samples collected during a dengue epidemic in jamaica.
Once the ns1 antigen igm igg antibody in patients serum was bound, conjugated monoclonal antibody was detected by adding the tetramethylbenzidine tmb substrate. Eden01g intended use the panbio dengue igg indirect elisa is for the qualitative detection of igg antibodies to dengue antigen serotypes 1, 2, 3 and 4 in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms and past exposure consistent with dengue fever. Repeat infection with a second type of dengue virus is thought to cause dengue hemorrhagic fever in about 10 percent of infected people. Igm antibodies by rapid visual immunochromatographybased test ict ezdx dengue combo agab from advy chemicals pvt. Eighty sera from patients with dengue virus infections, 24 sera from patients with japanese encephalitis je, and 78 sera from patients with nonflavivirus infections, such as malaria, typhoid. Indirect igg versus igg capture elisa for dengue diagnosis. A number of commercial elisa for dengue diagnosis have recently become available, though direct comparison between these assays have not been published. Panbio dengue igg capture elisa rapid point of care diagnostics.
We compared two generations of panbio brisbane, australia commercial kits for ns1 antigen capture for early diagnosis of dengue. Recommended test is ns1 antigen by elisa in the first 5 days of fever. Comparison of panbio dengue igm elisa assay with pentax dengue igm particle agglutination assay to evaluate factors affecting false positive results. The endemicity of dengue virus denv infection remains a major public health problem in lao pdr. Comparison of panbio dengue duo igm and igg capture elisa and. Assays for the serological diagnosis of dengue infections dengue igmcapture elisa panbio dengue iggindirect elisa panbio dengue ns1 antigen detection elisa panbio trainer. The test improvement resulted in a highly sensitive and speci. Comparison of seven commercial antigen and antibody enzyme. Thane, mumbai for dengue ns1, igg and igm as per manufacturers instructions.
Evaluation of a rapid immunochromatographic device for the. Antihuman igg antibodies are coated onto microtiter wells. The results obtained with individual sera in the panbio and mrl igm elisas. Dengue igg igm elisa kit parasitology antibody detection elisa kits. The performances of the mrl dengue fever virus immunoglobulin m igm capture enzymelinked immunosorbent assay elisa and the panbio dengue duo igm capture and igg capture elisa were compared. This study evaluated two newly introduced commercial tests for the detection of antibodies to dengue virus, the mrl diagnostics dengue fever virus igm capture elisa cypress, calif. The panbio dengue igm capture elisa is for the qualitative detection of igm antibodies to dengue antigen in serum, as an aid in the clinical laboratory. Evaluation of commercially available anti dengue virus immunoglobulin m tests iii dengue igm capture elisa pb igmcap panbio diagnostics, australia18 pathozyme dengue m pathozyme m omega diagnostics ltd. The panbio dengue igg capture elisa panbio diagnostics, brisbane, australia was used to determine the elevated level of igg antibodies to dengue virus serotypes 14 in a patients serum with secondary dengue virus infection. Comparison of two commercial elisa kits for the detection of. A comparative evaluation of dengue diagnostic tests based on. Evaluation of the mrl diagnostics dengue fever virus igm. Laboratory diagnosis of dengue with a single serum specimen obtained during the acute phase of the illness requires tests to detect igm antibodies to denv or the virus genome. Relevant identified uses of the substance or mixture and uses advised against not available not available safety data sheet sds according to regulation ec no 4532010 panbio dengue igm capture elisa doc.
Not for sale or distribution in the united states of america. The test improvement resulted in a highly sensitive and specific test suitable for use as a firstline test in the field. Evaluation of rapid ict in comparison with macelisa in. The inhouse igm elisa was performed as described previously lam et al. Identification of the substancemixture and of the companyundertaking cat. The laboratories using the assays from standard diagnostics dengue igg capture elisa and bioline dengue igmigg were free from. Dengue igm capture elisa is an enzyme linked immunosorbent assay for the qualitative detection of igm antibodies against dengue virus antigen in human serum. Bland and altman 95% limits of agreement ranged from. Pdf comparison of panbio dengue igm elisa assay with pentax. Tropicalmed free fulltext comparison of two commercial. Author summary dengue virus denv infection occurs throughout tropical and subtropical regions of the world where dengue is a major public health problem. Performance of commercial dengue ns1 elisa and molecular. Dengue antibodies do not confer immunity beyond 36 weeks to a second dengue type.